Oligomerization of the Sterile-2 G-Protein Coupled Receptor in Yeast Cells in the Presence and Absence of Alpha-Factor Pheromone Using Fluorescence Spectroscopy and Forster Resonance Energy Transfer Analysis
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G-protein-coupled receptors function as oligomers in vivo
Hormones, sensory stimuli, neurotransmitters and chemokines signal by activating G-protein-coupled receptors (GPCRs) [1]. Although GPCRs are thought to function as monomers, they can form SDS-resistant dimers, and coexpression of two non-functional or related GPCRs can result in rescue of activity or modification of function [2-10]. Furthermore, dimerization of peptides corresponding to the thi...
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The serotonin(1A) receptor is a representative member of the G-protein coupled receptor (GPCR) superfamily and serves as an important target in the development of therapeutic agents for neuropsychiatric disorders. Oligomerization of GPCRs is an important contemporary issue since it is believed to be a crucial determinant for cellular signaling. In this work, we monitored the oligomerization sta...
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